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Abstract #12335 Published in IGR 7-2
Effects of latanoprost eye drops on the proliferation of human Tenon's fibroblasts in tissue culture
Chen YH; Sun XHChinese Journal of Ophthalmology 2005; 41: 620-624
OBJECTIVE: To observe whether there is a direct effect of latanoprost eye drops on the proliferation of cultured human Tenon's capsule fibroblasts (HTCFs) in vitro. METHODS: Human Tenon's capsule fibroblasts were cultured in vitro. Latanoprost eye drops was serially diluted in order to make nine concentrations ranging from 5.00 microg/ml, 0.50 microg/ml, 0.05 microg/ml to 5 x 10-8 microg/ml. Fibroblasts growth was evaluated with MTT assay. Morphological changes were observed under phase contrast microscope. RESULTS: Majority of cultured human Tenon's capsule fibroblasts was dead at the concentration of Latanoprost (5 microg/ml), the A values were significantly decreased (P < 0.01). There were no significant changes of the A values (P > 0.05) in lower concentration (under 0.05 microg/ml) compared with control. there was no statistical difference in proliferation of HTCFs after latanoprost treatment for 24 hours, 48 hours, 72 hours. CONCLUSIONS: Latanoprost has the toxic effect on the fibroblast at higher concentrations, but there is no influence on the proliferation of HTCFs at the lower concentrations. LA: Chinese
Dr. Y.H. Chen, Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai 200031, China.
Classification:
11.4 Prostaglandins (Part of: 11 Medical treatment)