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Editors Selection IGR 22-3

Basic Research: Aqueous humor dynamics

Comment by Makoto Aihara on:

27733 Assessment of Aqueous Humor Dynamics in the Mouse by a Novel Method of Constant-Flow Infusion, Millar JC; Clark AF; Pang L-H, Investigative Ophthalmology and Visual Science, 2011; 52: 685-694

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The mouse remains important as a animal for clarifying the mechanism of diseases. Although in glaucoma, mouse eyes have been used because of their structural similarities to human eyes, the measurement of IOP and aqueous humor dynamics has been a big challenge because of the size of the mouse eye. These parameters had been previously measured in different eyes, but Millar et al. (97) recently developed the techniques to measure aqueous humor dynamics in the same eye of a mouse. One of the challenges of this study is that Fu-live and Fu-dead were equal and thus Fu was directly detectable in the dead eyes. The authors cleared this point by the classical FITC-dextran perfusion methods. Constant perfusion methods with several flow rates strengthened the data with subtle changes of values in the small eyes. Also, the development of the rebound tonometer for small animals and the unique measurement of Pe by previous studies enabled the authors to obtain the data of aqueous parameters in the same eyes. One important issue with most studies of aqueous humor dynamics, including this one, is that Fu is assumed to be pressure-independent. This point is still under debate with respect to the Goldmann equation. Another concern of this study is that one or two drops of tested drops applied to the mouse eye were too much in mouse eyes to extrapolate for primate or human studies. This paper is a step forward in the establishment of aqueous-humor measurements in the same animal eye and also to clarify the IOP-lowering mechanisms of drugs.

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